CAS:113231-05-3|(5S)-N-(5-Amino-1-carboxypentyl)iminodiacetic Acid

Catalogue Number:A603250
Chemical Name:(5S)-N-(5-Amino-1-carboxypentyl)iminodiacetic Acid
Synonyms:N2,N2-Bis(carboxymethyl)-L-lysine; Lysine-N,N-diacetic Acid; AB-NTA;
CAS Number:113231-05-3
Molecular Formula:C₁₀H₁₈N₂O₆
Appearance:White to Off-White Solid
Melting Point:>208°C (dec.)
Molecular Weight:262.26
Storage:-20°C
Solubility:Aqueous Acid (Slightly), Methanol (Very Slightly, Heated), Water (Sparingly, Son
Stability
Hygroscopic
Category:Building Blocks; Amino Acids;
Applications:A nitrilotriacetic acid derivative used as a metal chelating adsorbent for metal ion affinity chromatography. This method can be used for identification and rapid one-step purification of gene products expressed as fusion proteins with an oligo-histidine tag. The oligo-histidine tag serves as a high affinity binding sequence for the purification of fusion proteins via a metal chelating absorbent such as N-(5-Amino-1-carboxypentyl)iminodiacetic acid.Also soluble in DMSO:DMF 1:1 and DMSO:CH2Cl2 1:2
References:Hochuli, E., et al.: J. Chromatography, 411, 177 (1987), Meredith, G.D., et al.: Bioconjugate Chem., 15, 969 (2004)
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