CAS:205379-08-4|N-(5-Amino-1-carboxypentyl)iminodiacetic Acid Tri-t-butyl Ester

Catalogue Number:A603260
Chemical Name:N-(5-Amino-1-carboxypentyl)iminodiacetic Acid Tri-t-butyl Ester
Synonyms:N2,N2-Bis[2-(1,1-dimethylethoxy)-2-oxoethyl]-L-lysine 1,1-Dimethylethyl Ester
CAS Number:205379-08-4
Molecular Formula:C₂₂H₄₂N₂O₆
Appearance:Yellow Oil
Molecular Weight:430.58
Storage:-20°C
Solubility:Chloroform (Slightly), Methanol (Slightly)
Stability
Moisture Sensitive
Category:Research Tools; Amino Acids;
Applications:A nitrilotriacetic acid derivative used as a metal chelating adsorbent for metal ion affinity chromatography. This method can be used for identification and rapid one-step purification of gene products expressed as fusion proteins with an oligo-histidine tag. The oligo-histidine tag serves as a high affinity binding sequence for the purification of fusion proteins via a metal chelating absorbent such as N-(5-Amino-1-carboxypentyl)iminodiacetic acid.Also soluble in DMSO:DMF 1:1 and DMSO:CH2Cl2 1:2
References:Hochuli, E., et al.: J. of Chromatography, 411, 177 (1987)
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